
Linear regression

Exponential regression


Cytokine

R

R^{2}

p

A$\left(\frac{\mathit{pg}\mathbf{\times}\frac{\mathbf{1}}{\mathit{ml}}}{\mathbf{\mu}}\right)$

B$\left(\frac{\mathit{pg}}{\mathit{ml}}\right)$

R

R^{2}

p

K$\left(\frac{\mathbf{1}}{\mathit{\mu l}}\right)$

Y_{0}× 10^{−3}$\left(\frac{\mathit{pg}}{\mathit{ml}}\right)$


IL9

0.48

0.23

0.008

−163.46

7.7 × 10^{−3}

0.39

0.15

0.035

−0.04

5.6

IL10

0.40

0.16

0.027

−97.15

7.6 × 10^{−3}

0.56

0.32

0.001

−0.02

7.7

IL12p40

0.87

0.76

0.000

−317.36

12.0 × 10^{−3}

0.87

0.76

0.000

−0.05

12.0

IL12p70

0.71

0.50

0.000

−379.48

19.0 × 10^{−3}

0.74

0.54

0.000

−0.03

20.0

IL17A

0.38

0.15

0.037

−266.41

18.5 × 10^{−3}

0.11

0.01

0.547

−0.01

12.7

Eotaxin

0.65

0.42

0.000

−438.07

18.0 × 10^{−3}

0.74

0.55

0.000

−0.05

19.0

KC

0.83

0.70

0.000

−960.63

27.0 × 10^{−3}

0.91

0.83

0.000

−0.16

26.0

MCP1

0.91

0.83

0.000

−156.83

6.0 × 10^{−3}

0.91

0.82

0.000

−0.05

6.4

MIP1β

0.74

0.55

0.000

−278.96

11.0 × 10^{−3}

0.47

0.22

0.009

−0.04

7.6

RANTES

0.72

0.52

0.000

−20.70

0.9 × 10^{−3}

0.65

0.42

0.000

−0.04

0.8

 For cytokines that were significantly different between serum and heparin, the data were analyzed for a correlation between the concentration of the analyte and the amount of heparin added; R, R^{2}, pvalues, slopes (A) and intercepts (B) for the linear regressions and intercepts (y_{0}) and coefficients (K) for the exponential regressions are given. The calculations are based on the addition of 1 μl or 10 μl heparin (5,000 IU/ml). Where significant, the relationship between the specific cytokine concentration (pg/ml) and heparin (X) volume (μl) can be described as [Cyt] = B + A × X or $\left[\mathit{Cyt}\right]={\mathbf{y}}_{0}\times {\mathit{e}}^{\left(\mathit{K}\times \mathit{X}\right)}$.